Sonus complete (I) qRT-PCR analysis of c-JUN mRNA expression in 231.pLenti, 231.Src.sh.Scr, 231.Src.shEZH2 #3, and 231.Src.shEZH2 #4. n = 3. Data are means ± SEM. ****P < 0.0001, t test. (J) c-JUN promoter activity measured by dual-luciferase reporter assay in MDA-MB-231 cells transduced with lentiviruses harboring control vector (231.pLenti) or expressing EZH2 (231.EZH2) (left) and in 231.3C13.pLenti, 231.3C13.WT, 231.3C13.YF, and 231.3C13.YD cells (right).
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